By Mehmet Sengun Ozsoz
This ebook specializes in the fundamental electrochemical functions of DNA in quite a few parts, from easy ideas to the newest discoveries. The publication contains theoretical and experimental research of varied houses of nucleic acids, examine equipment, and a few promising purposes. the themes mentioned within the booklet contain electrochemical detection of DNA hybridization in line with latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interplay; nanoparticle-induced catalysis for DNA biosensing; uncomplicated phrases concerning electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; program of field-effect transistors to label loose electric DNA biosensor arrays; and electrochemical detection of nucleic acids utilizing branched DNA amplifiers. Read more...
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5 nM. This aptamer binds to the heparin-binding exosite of thrombin. RNA aptamers for thrombin have also been selected. Ikebukuro et al.  ﬁrst reported an electrochemical aptasensor for the detection of thrombin based on a sandwich-based assay. Two diﬀerent aptamers speciﬁc for thrombin were used: the 29mer thiolated aptamer and the 15-mer aptamer labeled with glucose dehydrogenase (GDH). The thiolated aptamer was immobilized onto gold electrodes; thrombin at diﬀerent concentrations and then the enzyme-labeled aptamer was added to the aptamer-modiﬁed electrodes.
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March 19, 2012 18:56 PSP Book - 9in x 6in Detection of Speciﬁc DNA Interactions of the metal complex indicator that binds to DNA (such as [Co(phen)3 ]3+ [14, 44–46]) or into enhancement of the voltammetric response of the negatively charged metal complex like [Fe(CN)6 ]3–/4– , which is repulsed by the negatively charged DNA layer depending on the degree of DNA damage [47, 48]. Change in the indicator electrochemical response depends on the portion of DNA damaged in the cleavage reaction. Similarly, a decrease in the charge transfer resistance at an impedimetric biosensor with hexacyanoferrate as the redox indicator in solution was used [47, 48].