By M. Gladwin, B. Tradler

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Sample text

T h e r e a d e r s h o u l d refer to t h e r u l e s g o v e r n i n g m i c r o s c o p e illumination. (27) P a r t i a l d a r k n e s s of t h e field c a n b e caused by the revolving nosepiece not being t u r n e d as_far a s , o r b e i n g p u s h e d b e y o n d , t h e s t o p n o t c h . T h e o b j e c t i v e is o n l y a t t h e c e n t r e w h e n t h e r e s i s t a n c e of t h e s t o p n o t c h is felt. Chief instrumental methods 31 (28) D i s p l a c e m e n t of t h e i m a g e w h e n t h e focus is v a r i e d .

O n e m a y choose a point source s u c h a s a h i g h i n t e n s i t y z i r c o n a r c , from w h i c h t h e i m a g e of t h e c i r c u l a r s o u r c e w o u l d b e p r o jected towards the microscope condenser t h r o u g h a v a r i a b l e focus z o o m d e v i c e . T h e c o n d e n s e r d i a p h r a g m r e t a i n s its u s u a l f u n c t i o n of r e g u l a t i n g t h e a p e r t u r e . T h i s t y p e of i l l u m i n a t i o n c a n a l s o b e o b t a i n e d b y t h e following o r i g i n a l p r o c e d u r e w h i c h gives v e r y g o o d r e s u l t s .

T h e dislike of m i c r o s c o p i s t s for t h i s m e t h o d d a t e s from t h e a p p e a r a n c e of s p i r a l f i l a m e n t b u l b s . T h e p r o j e c t i o n of t h e f i l a m e n t in t h e o b j e c t p l a n e is c o n f u s i n g , a n d gives rise to d i s t u r b i n g i r r e g u l a r i t i e s in i l l u m i n a t i o n w h i c h a r e e v e n m o r e d i s t u r b i n g . A n o t h e r d i s a d v a n t a g e lies in t h e fact t h a t it is i m p o s s i b l e to l i m i t t h e field i l l u m i n a t e d to t h a t o b s e r v e d .

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