By Russell C. Johnson

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Pugh. Urea utilization by Leptospira. Infect. Immun. 10^793-801, 1974. Kelly, R. Cultivation of Borrelia hermsi. Science 172:443-444, 1971. B. W. Watson. Biologically active endotoxins from Salmonella mutants dificient in O- and R-polysaccharides and heptose. J. Bacteriol. 94: 1320-1326, 1967. C. M. Jenkin. Isolation of an unusual positional iomer of hexadecenoic acid from a parasitic leptospire. Lipids 4^:166-167, 1969. P. C. Johnson. Lipids of the Spirochaetales: Comparison of the lipids of several 47 RUSSELL C.

C. Johnson. Lipids of the Spirochaetales: Comparison of the lipids of several 47 RUSSELL C. JOHNSON 26. 27. 28. 29. 30. 31. 32. members of the genera Spirochaeta Treponema and Leptospira. J. Bacteriol. 120:1268-1273, 1974. G. W. Scherp. Preparation and biological activities of endotoxins from oral bacteria. Infect. 108:304-310, 1961. Shinagawa, M. and R. Yanagawa. Isolation and characterization of a leptospiral type-specific antigen. Infect. Immun. 5^12-19, 1972. M. The isolation, cultivation and characterization of anaerobic tréponèmes.

1 μm. 30 THE BIOLOGY OF PARASITIC SPIROCHETES Fig. 20. Unclassified Leptospira (Canada BW-2), ΡΤΆ negatively-stained. Cell-free non-membrane-bound granules released from a single mechanically-disrupted cell illustrating their uniformity. 2 μρι. Fig. 21» Serotype icterohemorrhagiae, ΡΤΆ negativelystained. Degenerating cell with intracellular tubular structures reminiscent of mrhapidosomes" of phage-carrying microorganisms. Similar structures have been seen in deoxycholatetreated pomona. 2 μm. 31 A.

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