By Alfred Brown, Heidi Smith

Benson's Microbiological Applications has been the optimum of microbiology lab manuals for over 30 years. The self-contained, clearly-illustrated workouts and four-color layout makes this the appropriate lab guide. applicable for both a majors or non-majors lab path, this guide assumes no earlier natural chemistry has been taken.

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2 illustrates a second method, in which organisms are mixed in only a loopful of nigrosine instead Negative staining technique, using a spreader slide Benson: Microbiological Applications Lab Manual, Eighth Edition III. Microscope Slide Techniques 11. Negative Staining © The McGraw−Hill Companies, 2001 Negative Staining of a full drop. In this method the organisms are spread over a smaller area in the center of the slide with an inoculating needle. No spreader slide is used in this method. The third procedure (Woeste-Demchick’s method), which is not illustrated here, involves applying ink to a conventional smear with a black felt marking pen.

Needles are preferred, however, when making transfers in pure culture isolations and making stab cultures. 3 © The McGraw−Hill Companies, 2001 pure culture isolations, a needle is inserted into the center of a colony for the transfer. This technique is used, primarily, when working with mixed cultures. Plate Handling Media in plates must always be protected against contamination. To prevent exposure to air contamination, covers should always be left closed. 3. Flaming Procedures Inoculating loops or needles must be flamed in the same manner that you used when working with previous tubes.

Paecilomyces—yellowish-brown; elliptical microconidia. 10. Alternaria—dark greenish-black surface with gray periphery; black on reverse side; chains of macroconidia. 11. Bipolaris—black surface with grayish periphery; macroconidia shown. 52 © The McGraw−Hill Companies, 2001 10. The Fungi: Yeasts and Molds 1. Place the uncovered plate on the stage of your microscope and examine the edge of a colored colony with the low-power objective. Look for hyphal structure and spore arrangement. Ignore the white colonies since they generally lack spores and are difficult to identify.

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