By Klaus Aktories

It is a survey of good characterised and lately stumbled on bacterial protein pollutants. prime investigators of the respective pollutants evaluation many of the molecular mechanisms of motion, starting from toxin-induced ADP-ribosylation as much as membrane perforation by way of pore-forming pollutants. Thy additionally describe the implications on host body structure sooner than targeting strength purposes as phone organic and pharmacological instruments for learn and scientific purposes. unique descriptions of the technique comprise the engineering and use of converted and chimeric pollution for greater functionality. an outstanding creation to toxin constitution and capabilities, in addition to a invaluable resource of technique for researchers in molecular biology, pharmacology and experimental medication.

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Extra resources for Bacterial Toxins: Tools in Cell Biology and Pharmacology

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4 Assay 2: The Agmatine Assay This assay measures toxin-catalyzed transfer of ADP-ribose to agmatine, an analogue of arginine lacking the carboxyl moiety, using [14Cadenine]NAD. The reaction product, ['4C]ADP-ribosylagmatine,has a net charge of zero and is eluted from an AG 1-X2 anion-exchange column with water, while the substrate, [14C]NAD, is retained. ['4C]ADP-ribosylagmatineis quantified by liquid scintillation counting. Other simple guanidino compounds such as arginine methyl ester can also be utilized in this assay (Moss and Vaughan, 1977), provided a suitable method is used to isolate the ADP-ribosylated product.

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