By Corinne Whitby, Torben Lund Skovhus

Applied Microbiology and Molecular Biology in Oil box platforms addresses the foremost difficulties microbes reason in oil fields, (e.g. biocorrosion and souring) and the way valuable microbial actions could be exploited (e.g. MEOR and biofuels). The booklet describes theoretical and functional techniques to precise Molecular Microbiological equipment (MMM), and is written via top professionals within the box from either academia and undefined. The booklet describes how MMM will be utilized to faciliate greater administration of oil reservoirs and downstream techniques. The publication is leading edge in that it utilises actual business case reviews which provides helpful technical and clinical info to researchers, engineers and microbiologists operating with oil, fuel and petroleum systems.

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Extra info for Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009

Example text

Overall core properties are retrieved from a depth of 1154 m. The ambient temperature and pressure were 43◦ C and 120 bar, respectively. The core was salt saturated. M. van der Kraan et al. Archaea that are found in the core and to see if the different locations (outer shell to centre) in the core display differences in their microbial community. From the taken core segments genomic DNA was isolated (see Chapter 2). In this case, 16S rRNA gene fragments were amplified in a PCR reaction using primer pairs for Bacteria and Archaea, amplifying all the 16S rRNA genes of the different species present in the samples.

Van der Kraan et al. Moura A, Tacao M, Henriques I, Dias J, Ferreira P, Correia A (2009) Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR-DGGE analysis. Microbiol Res 164:560–569 Mueller RF, Nielsen PH (1996) Characterization of thermophilic consortia from two souring oil reservoirs. Appl Environ Microbiol 62:3083–3087 Nazina TN, Shestakova NM, Grigor’ian AA et al (2006) Phylogenetic diversity and activity of anaerobic microorganisms of high-temperature horizons of the Dagang Oilfield (China).

The GenBank database). Case Study 1: PCR-DGGE on an Oilfield Core Sample In this case study a PCR-DGGE analysis on 16S rRNA gene fragments has been performed on four different segments taken from an African oilfield core sample. Overall core properties are retrieved from a depth of 1154 m. The ambient temperature and pressure were 43◦ C and 120 bar, respectively. The core was salt saturated. M. van der Kraan et al. Archaea that are found in the core and to see if the different locations (outer shell to centre) in the core display differences in their microbial community.

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