By W. B. Hugo, J. B. Stenlake

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Further confirmation of the identity of an unknown organism may be made by means of an agglutinin absorption test. Briefly the unknown organism is added in excess to the agglutinating antiserum; during the agglutination process the antibody or antibodies will be absorbed from the serum. The mixture is now centrifuged and a sample of the serum from the clear supernatant layer is again tested against an authentic strain of the organism; agglutination should not now occur. It has been found that micro-organisms which are indistinguishable biochemically and morphologically may be quite distinct antigenically.

6th Symposium, Soc. gen. Microbiol. Ed. Spooner, E. T. C , and Stocker, B. A. D. Cambridge University Press, 1956 The Surface Structure of Bacteria. Rogers, H. J. In Biochemical Society Symposium No. 22. The structure and function of the membranes and surfaces of cells, p. 55. Ed. Bell, D. , and Grant, J. K. Cambridge University Press, 1963 Spore Formation and Germination as a Microbial Reaction to the Environment. Murrel, W. G. In Microbial Reaction to Environment. 11th Symposium, Soc. gen. , p.

One method of achieving these stepwise bursts of growth is to cool a growing culture from 37° to 4° C and then rewarm to 37°. The growth curves shown in Fig. 13 were obtained by such a method. 40 BACTERIAL GROWTH 6-8' I | 20 i , . 40 , 60 1 j — 80 TIME (minutes) F I G . 13. Synchronous growth in a nutrient broth culture of Escherichia coli, Results of two separate experiments are shown. (A. D. D. thesis. University of Nottingham) The above data have been obtained from what is called batch culture.

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